Association analysis of variant near ZNF389 with ewe cumulative production in three sheep breeds

experiments revealed the existence of eight and three single nucleotide polymorphisms (SNPs) at the promoter and 30-UTR of the goat LALBA gene respectively (Table S2 and Fig. S1). We also found eight additional putative polymorphic sites at exonic (all synonymous) and intronic regions. The potential functional significance of substitutions detected at the LALBA promoter was evaluated with the TFSEARCH software (http://www.cbrc.jp/research/db/TFSEARCH.html). This analysis showed that polymorphisms at SNPs c.-440C>T and c.-358C>T may destroy putative E2F (c.-440C>T, allele T) and p300 and CdxA (c.-358C>T, allele C) transcription factor binding sites. Analysis with UTRSCAN did not reveal any coincidence between SNPs c.*130C>T, c.*203C>T and c.*214C>T and 30-UTR functional motifs.